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total (t)-erk  (Wuhan Sanying Biotechnology)
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Wuhan Sanying Biotechnology total (t)-erk
(A) Western blot analysis of <t>p-ERK,</t> t-ERK <t>and</t> <t>β-actin.</t> H9c2 cells were incubated with Ang II (0 or 300 nmol/l) for up to 48 h. The bar graph shows semi-quantification of p-ERK/t-ERK expression. (B) Western blot analysis of p-ERK following pre-treatment with an ERK inhibitor. H9c2 cells were grown in culture and pre-incubated with an ERK inhibitor (PD; 20 µ mol/l) for 30 min followed by treatment with Ang II for 48 h. The bar graph shows semi-quantification of p-ERK/t-ERK expression. Data are presented as the mean ± SEM; n=3/group. * P<0.05. ERK, extracellular signal-regulated kinase; p, phosphorylated; PD, PD98059; t, total.
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(A) Western blot analysis of p-ERK, t-ERK and β-actin. H9c2 cells were incubated with Ang II (0 or 300 nmol/l) for up to 48 h. The bar graph shows semi-quantification of p-ERK/t-ERK expression. (B) Western blot analysis of p-ERK following pre-treatment with an ERK inhibitor. H9c2 cells were grown in culture and pre-incubated with an ERK inhibitor (PD; 20 µ mol/l) for 30 min followed by treatment with Ang II for 48 h. The bar graph shows semi-quantification of p-ERK/t-ERK expression. Data are presented as the mean ± SEM; n=3/group. * P<0.05. ERK, extracellular signal-regulated kinase; p, phosphorylated; PD, PD98059; t, total.

Journal: International Journal of Molecular Medicine

Article Title: Effects of thymoquinone against angiotensin II-induced cardiac damage in apolipoprotein E-deficient mice

doi: 10.3892/ijmm.2022.5119

Figure Lengend Snippet: (A) Western blot analysis of p-ERK, t-ERK and β-actin. H9c2 cells were incubated with Ang II (0 or 300 nmol/l) for up to 48 h. The bar graph shows semi-quantification of p-ERK/t-ERK expression. (B) Western blot analysis of p-ERK following pre-treatment with an ERK inhibitor. H9c2 cells were grown in culture and pre-incubated with an ERK inhibitor (PD; 20 µ mol/l) for 30 min followed by treatment with Ang II for 48 h. The bar graph shows semi-quantification of p-ERK/t-ERK expression. Data are presented as the mean ± SEM; n=3/group. * P<0.05. ERK, extracellular signal-regulated kinase; p, phosphorylated; PD, PD98059; t, total.

Article Snippet: Primary rabbit antibodies against phosphorylated (p)-extracellular signal-regulated kinase (ERK) (cat. no. 9102; 1:1,000; Cell Signaling Technology, Inc.), collagen I (cat. no. 14695-1-AP; 1:1,000; Wuhan Sanying Biotechnology), collagen III (cat. no. 22734-1-AP; 1:1,000; Wuhan Sanying Biotechnology), Nox4 (cat. no. 14347-1-AP; 1:1,000; Wuhan Sanying Biotechnology), p53 (cat. no. 10442-1-AP; 1:1,000; Wuhan Sanying Biotechnology), total (t)-ERK (cat. no. 11257-1-AP; 1:1,000; Wuhan Sanying Biotechnology) and β-actin (cat. no. 4970; 1:1,000; Cell Signaling Technology, Inc.) were used.

Techniques: Western Blot, Incubation, Expressing

(A) Western blot analysis of collagen I and III, Nox4 and p53 in H9c2 cells incubated with Ang II (300 nmol/l) and ERK inhibitor. (B) Bar graph shows semi-quantification of the protein expression levels of collagen I and III, Nox4 and p53 in H9c2 cells. H9c2 cells were grown in culture and pre-incubated with an ERK inhibitor (PD98059; 20 µ mol/l) for 30 min followed by treatment with Ang II for 48 h. Data are presented as the mean ± SEM; n=3/group. * P<0.05. ERK, extracellular signal-regulated kinase; p, phosphorylated; PD, PD98059; t, total.

Journal: International Journal of Molecular Medicine

Article Title: Effects of thymoquinone against angiotensin II-induced cardiac damage in apolipoprotein E-deficient mice

doi: 10.3892/ijmm.2022.5119

Figure Lengend Snippet: (A) Western blot analysis of collagen I and III, Nox4 and p53 in H9c2 cells incubated with Ang II (300 nmol/l) and ERK inhibitor. (B) Bar graph shows semi-quantification of the protein expression levels of collagen I and III, Nox4 and p53 in H9c2 cells. H9c2 cells were grown in culture and pre-incubated with an ERK inhibitor (PD98059; 20 µ mol/l) for 30 min followed by treatment with Ang II for 48 h. Data are presented as the mean ± SEM; n=3/group. * P<0.05. ERK, extracellular signal-regulated kinase; p, phosphorylated; PD, PD98059; t, total.

Article Snippet: Primary rabbit antibodies against phosphorylated (p)-extracellular signal-regulated kinase (ERK) (cat. no. 9102; 1:1,000; Cell Signaling Technology, Inc.), collagen I (cat. no. 14695-1-AP; 1:1,000; Wuhan Sanying Biotechnology), collagen III (cat. no. 22734-1-AP; 1:1,000; Wuhan Sanying Biotechnology), Nox4 (cat. no. 14347-1-AP; 1:1,000; Wuhan Sanying Biotechnology), p53 (cat. no. 10442-1-AP; 1:1,000; Wuhan Sanying Biotechnology), total (t)-ERK (cat. no. 11257-1-AP; 1:1,000; Wuhan Sanying Biotechnology) and β-actin (cat. no. 4970; 1:1,000; Cell Signaling Technology, Inc.) were used.

Techniques: Western Blot, Incubation, Expressing

(A) Western blot analysis of p-ERK in heart tissue. (B) Bar graph shows the semi-quantification of the expression levels of p-ERK in heart tissue. (C) Western blot analysis of p-ERK following pre-treatment of cells with an ERK inhibitor and TQ. (D) Bar graph shows semi-quantification of the expression levels of collagen I and III, Nox4 and p53 in H9c2 cells. H9c2 cells were cultured and pre-incubated with an ERK inhibitor (PD; 20 µ mol/l) for 30 min followed by treatment with Ang II for 48 h. For TQ treatment, cells were grown to 80% confluence and then incubated with TQ at the indicated concentration (20 µ mol/l) for 24 h. For PD + Ang II + TQ group, the cells were pre-incubated with PD and then treatment with Ang II and TQ as previously mentioned. DMSO treatment (final concentration, 0.1%) was used as a sham control. Data are presented as the mean ± SEM; n=3/group. * P<0.05, ** P<0.01. ERK, extracellular signal-regulated kinase; p, phosphorylated; PD, PD98059; t, total; TQ, thymoquinone.

Journal: International Journal of Molecular Medicine

Article Title: Effects of thymoquinone against angiotensin II-induced cardiac damage in apolipoprotein E-deficient mice

doi: 10.3892/ijmm.2022.5119

Figure Lengend Snippet: (A) Western blot analysis of p-ERK in heart tissue. (B) Bar graph shows the semi-quantification of the expression levels of p-ERK in heart tissue. (C) Western blot analysis of p-ERK following pre-treatment of cells with an ERK inhibitor and TQ. (D) Bar graph shows semi-quantification of the expression levels of collagen I and III, Nox4 and p53 in H9c2 cells. H9c2 cells were cultured and pre-incubated with an ERK inhibitor (PD; 20 µ mol/l) for 30 min followed by treatment with Ang II for 48 h. For TQ treatment, cells were grown to 80% confluence and then incubated with TQ at the indicated concentration (20 µ mol/l) for 24 h. For PD + Ang II + TQ group, the cells were pre-incubated with PD and then treatment with Ang II and TQ as previously mentioned. DMSO treatment (final concentration, 0.1%) was used as a sham control. Data are presented as the mean ± SEM; n=3/group. * P<0.05, ** P<0.01. ERK, extracellular signal-regulated kinase; p, phosphorylated; PD, PD98059; t, total; TQ, thymoquinone.

Article Snippet: Primary rabbit antibodies against phosphorylated (p)-extracellular signal-regulated kinase (ERK) (cat. no. 9102; 1:1,000; Cell Signaling Technology, Inc.), collagen I (cat. no. 14695-1-AP; 1:1,000; Wuhan Sanying Biotechnology), collagen III (cat. no. 22734-1-AP; 1:1,000; Wuhan Sanying Biotechnology), Nox4 (cat. no. 14347-1-AP; 1:1,000; Wuhan Sanying Biotechnology), p53 (cat. no. 10442-1-AP; 1:1,000; Wuhan Sanying Biotechnology), total (t)-ERK (cat. no. 11257-1-AP; 1:1,000; Wuhan Sanying Biotechnology) and β-actin (cat. no. 4970; 1:1,000; Cell Signaling Technology, Inc.) were used.

Techniques: Western Blot, Expressing, Cell Culture, Incubation, Concentration Assay